Caltech Nanofabrication Group

  • Increase font size
  • Default font size
  • Decrease font size
Home Research Microfluidics/Biomedical

Microfluidic Polymerase Chain Reaction

Research Area: Microfluidics/Biomedical Year: 2008
Type of Publication: Article Keywords: bioMEMS, DNA, heat exchangers, microfluidics, molecular biophysics, thermocouples
Authors: George Maltezos; A. Gomez; J. F. Zhong; F. A. Gomez; Axel Scherer
Journal: Applied Physics Letters Volume: 93
Number: 24 Pages: 243901
Month: December
Abstract:
We implement microfluidic technology to miniaturize a thermal cycling system for amplifying DNA fragments. By using a microfluidic thermal heat exchanger to cool a Peltier junction, we have demonstrated rapid heating and cooling of small volumes of solution. We use a miniature K-type thermocouple to provide a means for in situ sensing of the temperature inside the microrefrigeration system. By combining the thermocouple, two power supplies controlled by a relay system, and computer automation, we reproduce the function of a commercial polymerase chain reaction thermal cycler and demonstrate amplification of a DNA sample of about 1000 base pairs.
Full text:
PDF: Caltech only
Online version (publisher)

Contact

Administrative and Financial Contact

Kate Finigan
MC 200-79, Caltech
1200 E California Blvd
Pasadena, CA 91125

Office:  215 Powell-Booth
Phone:  626.395.4585
Fax: 626.577.8442
Email: kate@caltech.edu

Login