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A Microfabricated Device for Sizing and Sorting DNA Molecules

Research Area: Microfluidics/Biomedical Year: 1999
Type of Publication: Article Keywords: microfluidics, electrophoresis, soft lithography, single-molecule DNA sizing
Authors: Chou, Hou-Pu; Spence, C.; Scherer, Axel; Quake, S. R.
Journal: Proceedings of the National Academy of Sciences Volume: 96
Number: 1 Pages: 11-13
Month: January
We have demonstrated a microfabricated single-molecule DNA sizing device. This device does not depend on mobility to measure molecule size, is 100 times faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. Here we describe the fabrication and use of the single-molecule DNA sizing device for sizing and sorting DNA restriction digests and ladders spanning 2–200 kbp.
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