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Microfluidic Polymerase Chain Reaction

Research Area: Microfluidics/Biomedical Year: 2008
Type of Publication: Article Keywords: bioMEMS, DNA, heat exchangers, microfluidics, molecular biophysics, thermocouples
Authors: Maltezos, George; Gomez, A.; Zhong, J. F.; Gomez, F. A.; Scherer, Axel
Journal: Applied Physics Letters Volume: 93
Number: 24 Pages: 243901
Month: December
We implement microfluidic technology to miniaturize a thermal cycling system for amplifying DNA fragments. By using a microfluidic thermal heat exchanger to cool a Peltier junction, we have demonstrated rapid heating and cooling of small volumes of solution. We use a miniature K-type thermocouple to provide a means for in situ sensing of the temperature inside the microrefrigeration system. By combining the thermocouple, two power supplies controlled by a relay system, and computer automation, we reproduce the function of a commercial polymerase chain reaction thermal cycler and demonstrate amplification of a DNA sample of about 1000 base pairs.
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